منابع مشابه
Rapid Development of Nucleic Acid Diagnostics
There has been a significant increase, fueled by technologies from the human genome project, in the availability of nucleic acid sequence information for viruses and bacteria. This paper presents a computer-assisted process that begins with nucleic acid sequence information and produces highly specific pathogen signatures. When combined with instrumentation using the polymerase chain reaction, ...
متن کاملOne-step nucleic acid amplification for intraoperative detection of lymph node metastasis in breast cancer patients.
PURPOSE Detection of sentinel lymph node (SLN) metastasis in breast cancer patients has conventionally been determined by intraoperative histopathologic examination of frozen sections followed by definitive postoperative examination of permanent sections. The purpose of this study is to develop a more efficient method for intraoperative detection of lymph node metastasis. EXPERIMENTAL DESIGN ...
متن کاملAdvances in Single-molecule Nucleic Acid Sequencing
The ability to quickly and accurately obtain sequence information from single molecules of DNA and RNA has far-reaching implications for our understanding of biology. In the work presented here, we have made several advances in the area of single-molecule DNA and RNA sequencing. Specifically, in attempting to increase the read length of DNA polymerase, we have assayed several custom synthesized...
متن کاملAdvances in Nucleic Acid Detection and Quantification
The last decade has seen many changes in molecular biology at the bench, as we have moved away from a primary goal of cataloguing genes andmRNA towards techniques that detect and quantify nucleic acid molecules even within single cells. With the invention of the polymerase chain reaction (PCR), a nucleic acid sequence could now be amplified to generate a large number of identical copies, and th...
متن کاملFluorescent hybridization probes for nucleic acid detection.
Due to their high sensitivity and selectivity, minimum interference with living biological systems, and ease of design and synthesis, fluorescent hybridization probes have been widely used to detect nucleic acids both in vivo and in vitro. Molecular beacons (MBs) and binary probes (BPs) are two very important hybridization probes that are designed based on well-established photophysical princip...
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ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2008
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-008-2360-x